منابع مشابه
Vortex-assisted DNA delivery.
Electroporation is one of the most widely used methods to deliver exogenous DNA payloads into cells, but a major limitation is that only a small fraction of the total membrane surface is permeabilized. Here we show how this barrier can be easily overcome by harnessing hydrodynamic effects associated with Dean flows that occur along curved paths. Under these conditions, cells are subjected to a ...
متن کاملSonoporation: mechanical DNA delivery by ultrasonic cavitation.
Development of nonviral gene transfer methods would be a valuable addition to the gene-therapy armamentarium, particularly for localized targeting of specific tissue volumes. Ultrasound can produce a variety of nonthermal bioeffects via acoustic cavitation including DNA delivery. Cavitation bubbles may induce cell death or transient membrane permeabilization (sonoporation) on a single cell leve...
متن کاملCellular delivery of enzyme-loaded DNA origami.
In this communication, we show that active enzymes can be delivered into HEK293 cells in vitro when they are attached to tubular DNA origami nanostructures. We use bioluminescent enzymes as a cargo and monitor their activity from a cell lysate. The results show that the enzymes stay intact and retain their activity in the transfection process. The method is highly modular, which makes it a comp...
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Vanessa Bastos Pereira; Marcela Santiago Pacheco Azevedo; Tessália Diniz Luerce Saraiva; Bianca Mendes Souza; Meritxell Zurita Turk; Camila Prósperi De Castro; Pamela Mancha Agresti; Fernanda Alvarenga Lima; Vasco Azevedo; Daniela Santos Pontes and Anderson Miyoshi Laboratório de Genética Celular e Molecular, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Ho...
متن کاملAmphiphile-DNA complexes: adsorption, delivery and release
A theory is presented which quantitatively accounts for the cooperative adsorption of cationic surfactants to anionic polyelectrolyte [1,2]. For high salt concentrations, we find that the critical adsorption concentration (CAC) is a bilinear function of the polyion monomer and salt concentrations, with the coefficients dependent only on the type of surfactant used. Motivated by the theory, we h...
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ژورنال
عنوان ژورنال: Nature Reviews Neuroscience
سال: 2001
ISSN: 1471-003X,1471-0048
DOI: 10.1038/35104008